Hi, I'm trying to culture L. pneumophila in liquid culture using ACES buffered yeast extract broth (10g/L yeast extract, 0.4g/L L-cystein, 0.25g/L ferric pyrophosphate). Medium is filter sterilized.
4 Buffered Charcoal Yeast Extract (BCYE) Agar; 5 Cary-Blair Transport Medium; 6 Cetrimide Agar; 7 Chocolate Agar; 8 Cysteine Lactose Electrolyte Deficient (CLED) Agar; 9 Eosin Methylene Blue (EMB) Agar; 10 Hektoen Enteric (HE) Agar; 11 MacConkey Agar; 12 Mannitol Salt Agar (MSA) 13 Modified Thayer-Martin Agar; 14 Mueller Hinton Agar (MHA) 15 The recovery of Legionella micdadei and L. bozemanii serogroups 1 and 2 from infected guinea pig spleens was evaluated by using two culture media: buffered charcoal yeast extract agar with 0.1% alpha-ketoglutarate (BCYE alpha) and the same medium supplemented with 1% bovine serum albumin (ABCYE alpha). At the lowest dilution of spleen tissue (10(-1)), recovery of all strains of L. micdadei and BCYE (Buffered Charcoal Yeast Extract) Selective Agar with GVPC antibiotics, for Legionella, 26ml fill, 15x100mm plate (Buffered Charcoal Yeast Extract Agar with Glycine, Vancomycin, Polymyxin, and Cycloheximide) For the selective isolation of Legionella spp A patient with chronic osteomyelitis caused by Brucella abortus had negative agglutination titers. Because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (BCYE) agar. Buffered Charcoal Yeast Extract (BCYE) Feeley et al in 1978 developed a medium to isolate Legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract, and replacing starch with activated charcoal and named it as Charcoal Yeast Extract (CYE) Agar. Honeywell Fluka Buffered Charcoal Yeast Extract Agar (Base) 500G PLASTIC BOTTLE Buffered Charcoal Yeast Extract listed as BCYE. Buffered Charcoal Yeast Extract - How is Buffered Charcoal Yeast Extract abbreviated? https://acronyms
Colonization of Francisella tularensis on various media, namely: (A) buffered charcoal yeast extract; (B) chocolate agar medium; (C) sheep’s blood agar; (D) cysteine heart agar. Pathogenicity: F. tularensis has two main pathogenic serotypes: Subspecies tularenis (type-A) and subspecies holarctica (type-B). Both types possess a thin
Charcoal Yeast Extract (CYE) Agar was developed by Feely et al. in 1979 as a modification of an existing medium, F-G Agar.1,2 They replaced the starch in the F-G agar with activated charcoal and substituted yeast extract for casein hydrolysate, resulting in better recovery of L. pneumophila. Hi, I'm trying to culture L. pneumophila in liquid culture using ACES buffered yeast extract broth (10g/L yeast extract, 0.4g/L L-cystein, 0.25g/L ferric pyrophosphate). Medium is filter sterilized. The plates, upon spread of lysed blood sediment from the Isolator tube, were incubated for 5 days with 5% carbon dioxide. Numerous colonies grew on buffered charcoal yeast extract agar (top right), fewer and smaller colonies on the chocolate agar (top left and lower right), and none on blood agar (lower left).
Yeast Extract provides sources of nitrogen, carbon, and vitamins in BCYE Agar. Activated Charcoal decomposes hydrogen peroxide and other toxic products. ACES Buffer was added to formulation to get a stable pH for optimal growth. α-Ketoglutarate stimulates organism growth. Agar is the solidifying agent. L-Cysteine, in the supplement, is an
Allow sample to cool to room temperature before reading pH. Keep charcoal in suspension when dispensing. For solid medium, add 17 grams of Agar to base medium above. **Note: Medium should be wrapped in foil to protect from light. **Note: Be sure to allow solid products adequate time to dry (at least two days). Agar